Prevention of experimental choroidal neovascularization and resolution of active lesions by VEGF trap in nonhuman primates

Arch Ophthalmol. 2011 Aug;129(8):1042-52. doi: 10.1001/archophthalmol.2011.210.

ABSTRACT

OBJECTIVE: To evaluate the efficacy of systemic and intravitreous administration of VEGF Trap (aflibercept) in a nonhuman primate model of choroidal neovascularization (CNV).

METHODS: VEGF Trap treatment on laser-induced CNV was evaluated in 48 adult cynomolgus monkeys. In the prevention arms of the study, VEGF Trap was administered by intravenous injection (3 or 10 mg/kg weekly) or intravitreous injection (50, 250, or 500 μg/eye every 2 weeks) beginning before laser injury. In the treatment arm, a single intravitreous injection (500 μg) was given 2 weeks following laser injury. Laser-induced lesions were scored from grade 1 (no hyperfluorescence) to grade 4 (clinically relevant leakage). Representative lesions were evaluated histologically.

RESULTS: Grade 4 leakage developed at 32.4% and 45.4% of the laser sites in animals receiving intravitreous or intravenous administration of placebo at 2 weeks following laser injury, respectively. In contrast, the development of grade 4 lesions was completely or nearly completely prevented in all groups receiving intravenous or intravitreous injections of VEGF Trap. A single intravitreous injection of VEGF Trap (500 μg) administered following the development of CNV reduced the frequency of grade 4 lesions from 44.4% to 0% within 14 days of treatment. Intravitreous VEGF Trap was well tolerated with either no or only mild ocular inflammation. Histological evaluation showed decreased scores for morphologic features of tissue proliferation in the VEGF Trap prevention groups.

CONCLUSIONS: VEGF Trap prevented the development of clinically relevant CNV leakage when administered at the lowest doses tested. Moreover, a single intravitreous injection induced inhibition of active CNV leakage.

CLINICAL RELEVANCE: The animal model used in this study has an established track record as a predictor of pharmacologic efficacy of antineovascular drugs in humans having the neovascular, or wet, form of age-related macular degeneration.

PMID:21825187 | DOI:10.1001/archophthalmol.2011.210

Description

CONCLUSIONS: VEGF Trap prevented the development of clinically relevant CNV leakage when administered at the lowest doses tested. Moreover, a single intravitreous injection induced inhibition of active CNV leakage.

pubmed:21825187
https://pubmed.ncbi.nlm.nih.gov/21825187/?utm_source=curl&utm_medium=rss&utm_campaign=None&utm_content=1D1U_2QMDWYKn_cXR-rtstRmreLn3QCrs1Teh4Y_EhqdhU3Q7j&fc=None&ff=20240902234023&v=2.18.0.post9+e462414
Published Date
2011-08-10
Associated Team Member
Arch Ophthalmol
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129(8):1042-52
2011
08
T Michael Nork, Richard R Dubielzig, Brian J Christian, Paul E Miller, Jacqueline M Miller, Jingtai Cao, Edward P Zimmer, Stanley J Wiegand
Approved Therapy
Investigation Type
Development Phase
Therapy Class