Yelena Krakova has extensive technical expertise in electrophysiological testing on a wide variety of animal models including rodents, rabbits, dogs and non human primates. At OSOD, she provides support for the development and implementation of mfERG system testing methods, signal quality, data analysis, and output presentation formats. She is also an integral member of the Optical Coherence Tomography (OCT) team, involved in both image acquisition and analysis. She is also skilled in non-contact specular microscopy and ultrasound pachymetry and provides administrative support for many of OSOD's vision scientists. When needed, she is also responsible for providing equipment maintenance to proactively avoid issues in GLP and non-GLP studies and enhance study efficiency. Prior to joining OSOD, Ms. Krakova's focus was on electrophysiology and ocular toxicology, undertaking graduate research in a neural engineering vision laboratory to help pioneer the development and testing of a novel product for the research and diagnosis of retinal diseases. Additionally, she has six years of laboratory experience assisting the development of accurate testing procedures and data collection methods to help deliver high quality study data with prompt turnaround.
Spatial differences in corneal electroretinogram potentials measured in rat with a contact lens electrode array.
Doc Ophthalmol. 2014 Dec;129(3):151-66
Authors: Krakova Y, Tajalli H, Thongpang S, Derafshi Z, Ban T, Rahmani S, Selner AN, Al-Tarouti A, Williams JC, Hetling JR
PURPOSE: It has been known for several decades that the magnitude of the corneal electroretinogram (ERG) varies with position on the eye surface, especially in the presence of focal or asymmetric stimuli or retinal lesions. However, this phenomenon has not been well-characterized using simultaneous measurements at multiple locations on the cornea. This work provides the first characterization of spatial differences in the ERG across the rat cornea.
METHODS: A contact lens electrode array was employed to record ERG potentials at 25 corneal locations simultaneously following brief full-field flash stimuli in normally sighted Long-Evans rats. These multi-electrode electroretinogram (meERG) responses were analyzed for spatial differences in a-wave and b-wave amplitudes and implicit times.
RESULTS: Spatially distinct ERG potentials could be recorded reliably. Comparing relative amplitudes across the corneal locations suggested a slight non-uniform distribution when using full-field, near-saturating stimuli. Amplitudes of a- and b-waves were approximately 3 % lower in the inferior quadrant than in the superior quadrant of the cornea.
CONCLUSIONS: The present results comprise the start of the first normative meERG database for rat eyes and provide a basis for comparison of results from eyes with functional deficit. Robust measures of spatial differences in corneal potentials will also support optimization and validation of computational source models of the ERG. To fully utilize the information contained in the meERG data, a detailed understanding of the roles of the many determinants of local corneal potentials will eventually be required.
PMID: 25266461 [PubMed - indexed for MEDLINE]