In Vitro Assays

OSOD laboratories could provide support for the following in vitro/ ex vivo assays. If the assay you seek is not listed here, please let us know and it is likely that we can provide it.

  • Live dead cell assays. These are used for assessing viability on various cell lines.  Two-color fluorescence assays provide accurate counts of the numbers of live and dead cells. 
  • Evaluation of toxicity, proliferation, and migration of retinal endothelial cells, pericytes, and astrocytes from C57BL/6J mice and human retinal pigment epithelial (RPE) cells following in vitro exposure.
  • Tube formation assays. These provide a model for the study of how endothelial cells work to disrupt neighboring membranes, directionally align and migrate toward a stimulus where additional cells are produced to create new vessels, and ultimately a network of three-dimensional vascular structures.  Understanding of inhibition and how induction of new blood vessels impact existing vasculature through angiogenesis may provide clues to understanding developmental processes associated with diabetic retinopathy, tumors, and wound healing.
  • Ocular penetration assays.  These are being used to assess the beneficial and adverse effects of inflammation on the intraocular penetration of various compounds on ocular infections, corneal wound healing, and intraocular pressure.